owls owls model 210 Search Results


owls 210 instrument  (MicroVacuum Ltd)
90
MicroVacuum Ltd owls 210 instrument
Owls 210 Instrument, supplied by MicroVacuum Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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night owl ccd camera lb 981  (Berthold Technologies)
90
Berthold Technologies night owl ccd camera lb 981
Night Owl Ccd Camera Lb 981, supplied by Berthold Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tae  (Thermo Fisher)
97
Thermo Fisher tae
Tae, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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optical device fabrication  (Nanosensors Inc)
90
Nanosensors Inc optical device fabrication
Optical Device Fabrication, supplied by Nanosensors Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sound isolation chamber  (Industrial Acoustics Co Inc)
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Industrial Acoustics Co Inc sound isolation chamber
Sound Isolation Chamber, supplied by Industrial Acoustics Co Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna ladder  (Thermo Fisher)
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Thermo Fisher dna ladder
Figure 3. Oxidized RBC and DSRBC induce apoptosis in J774A.1 MP cells. J774A.1 MP remained untreated (control) or were incubated with oxi- dized RBC or DSRBC at the MP:RBC ratio 1:100. Camptothecin was used as a positive control for apoptosis. After 48 h, cell viability was assayed by TUNEL staining (A and B), <t>DNA</t> migration on agarose gel (C and D), or Hoechst/PI staining (E). (A) Characteristic density plots obtained for control (left panel) and oxidized RBC (MP:RBC ratio 1:100, right panel)-treated J774A.1 MP. R1 gate represents the percentage of TUNEL cells (i.e., apoptotic cells). SSC, Side-scatter. (B) Impact of camptothecin, oxidized RBC, and DSRBC on the percentages of TUNEL J774A.1 MP. (C) DNA profiles of control (Lane 2), oxidized RBC (Lane 3), or camptothecin (Lane 4)-treated J774A.1 MP; Lanes 1 and 5, DNA ladder. (D) DNA profiles of control (Lane 2), DSRBC (Lane 3), or camptothecin (Lane 4)-treated J774A.1 MP. Lanes 1 and 5: DNA ladder. (E) Hoechst/ PI-stained J774A.1 MP analyzed by fluorescence microscopy on cytospin slides. Columns 1–3 represent transmission light images, Hoechst fluores- cence, and PI fluorescence, respectively. Triangles indicate typical early-apoptosic cells and arrows identify characteristic late-apoptotic cells. Data are representative of two independent experiments showing the mean sd from quadruplicate cultures of one experiment.
Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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owls model owls110  (MicroVacuum Ltd)
90
MicroVacuum Ltd owls model owls110
Figure 3. Oxidized RBC and DSRBC induce apoptosis in J774A.1 MP cells. J774A.1 MP remained untreated (control) or were incubated with oxi- dized RBC or DSRBC at the MP:RBC ratio 1:100. Camptothecin was used as a positive control for apoptosis. After 48 h, cell viability was assayed by TUNEL staining (A and B), <t>DNA</t> migration on agarose gel (C and D), or Hoechst/PI staining (E). (A) Characteristic density plots obtained for control (left panel) and oxidized RBC (MP:RBC ratio 1:100, right panel)-treated J774A.1 MP. R1 gate represents the percentage of TUNEL cells (i.e., apoptotic cells). SSC, Side-scatter. (B) Impact of camptothecin, oxidized RBC, and DSRBC on the percentages of TUNEL J774A.1 MP. (C) DNA profiles of control (Lane 2), oxidized RBC (Lane 3), or camptothecin (Lane 4)-treated J774A.1 MP; Lanes 1 and 5, DNA ladder. (D) DNA profiles of control (Lane 2), DSRBC (Lane 3), or camptothecin (Lane 4)-treated J774A.1 MP. Lanes 1 and 5: DNA ladder. (E) Hoechst/ PI-stained J774A.1 MP analyzed by fluorescence microscopy on cytospin slides. Columns 1–3 represent transmission light images, Hoechst fluores- cence, and PI fluorescence, respectively. Triangles indicate typical early-apoptosic cells and arrows identify characteristic late-apoptotic cells. Data are representative of two independent experiments showing the mean sd from quadruplicate cultures of one experiment.
Owls Model Owls110, supplied by MicroVacuum Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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horizontal electrophoresis apparatus  (Thermo Fisher)
99
Thermo Fisher horizontal electrophoresis apparatus
Figure 3. Oxidized RBC and DSRBC induce apoptosis in J774A.1 MP cells. J774A.1 MP remained untreated (control) or were incubated with oxi- dized RBC or DSRBC at the MP:RBC ratio 1:100. Camptothecin was used as a positive control for apoptosis. After 48 h, cell viability was assayed by TUNEL staining (A and B), <t>DNA</t> migration on agarose gel (C and D), or Hoechst/PI staining (E). (A) Characteristic density plots obtained for control (left panel) and oxidized RBC (MP:RBC ratio 1:100, right panel)-treated J774A.1 MP. R1 gate represents the percentage of TUNEL cells (i.e., apoptotic cells). SSC, Side-scatter. (B) Impact of camptothecin, oxidized RBC, and DSRBC on the percentages of TUNEL J774A.1 MP. (C) DNA profiles of control (Lane 2), oxidized RBC (Lane 3), or camptothecin (Lane 4)-treated J774A.1 MP; Lanes 1 and 5, DNA ladder. (D) DNA profiles of control (Lane 2), DSRBC (Lane 3), or camptothecin (Lane 4)-treated J774A.1 MP. Lanes 1 and 5: DNA ladder. (E) Hoechst/ PI-stained J774A.1 MP analyzed by fluorescence microscopy on cytospin slides. Columns 1–3 represent transmission light images, Hoechst fluores- cence, and PI fluorescence, respectively. Triangles indicate typical early-apoptosic cells and arrows identify characteristic late-apoptotic cells. Data are representative of two independent experiments showing the mean sd from quadruplicate cultures of one experiment.
Horizontal Electrophoresis Apparatus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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owls 210  (Biacore)
86
Biacore owls 210
Figure 3. Oxidized RBC and DSRBC induce apoptosis in J774A.1 MP cells. J774A.1 MP remained untreated (control) or were incubated with oxi- dized RBC or DSRBC at the MP:RBC ratio 1:100. Camptothecin was used as a positive control for apoptosis. After 48 h, cell viability was assayed by TUNEL staining (A and B), <t>DNA</t> migration on agarose gel (C and D), or Hoechst/PI staining (E). (A) Characteristic density plots obtained for control (left panel) and oxidized RBC (MP:RBC ratio 1:100, right panel)-treated J774A.1 MP. R1 gate represents the percentage of TUNEL cells (i.e., apoptotic cells). SSC, Side-scatter. (B) Impact of camptothecin, oxidized RBC, and DSRBC on the percentages of TUNEL J774A.1 MP. (C) DNA profiles of control (Lane 2), oxidized RBC (Lane 3), or camptothecin (Lane 4)-treated J774A.1 MP; Lanes 1 and 5, DNA ladder. (D) DNA profiles of control (Lane 2), DSRBC (Lane 3), or camptothecin (Lane 4)-treated J774A.1 MP. Lanes 1 and 5: DNA ladder. (E) Hoechst/ PI-stained J774A.1 MP analyzed by fluorescence microscopy on cytospin slides. Columns 1–3 represent transmission light images, Hoechst fluores- cence, and PI fluorescence, respectively. Triangles indicate typical early-apoptosic cells and arrows identify characteristic late-apoptotic cells. Data are representative of two independent experiments showing the mean sd from quadruplicate cultures of one experiment.
Owls 210, supplied by Biacore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gel electrophoresis systems  (Fisher Scientific)
90
Fisher Scientific gel electrophoresis systems
Figure 3. Oxidized RBC and DSRBC induce apoptosis in J774A.1 MP cells. J774A.1 MP remained untreated (control) or were incubated with oxi- dized RBC or DSRBC at the MP:RBC ratio 1:100. Camptothecin was used as a positive control for apoptosis. After 48 h, cell viability was assayed by TUNEL staining (A and B), <t>DNA</t> migration on agarose gel (C and D), or Hoechst/PI staining (E). (A) Characteristic density plots obtained for control (left panel) and oxidized RBC (MP:RBC ratio 1:100, right panel)-treated J774A.1 MP. R1 gate represents the percentage of TUNEL cells (i.e., apoptotic cells). SSC, Side-scatter. (B) Impact of camptothecin, oxidized RBC, and DSRBC on the percentages of TUNEL J774A.1 MP. (C) DNA profiles of control (Lane 2), oxidized RBC (Lane 3), or camptothecin (Lane 4)-treated J774A.1 MP; Lanes 1 and 5, DNA ladder. (D) DNA profiles of control (Lane 2), DSRBC (Lane 3), or camptothecin (Lane 4)-treated J774A.1 MP. Lanes 1 and 5: DNA ladder. (E) Hoechst/ PI-stained J774A.1 MP analyzed by fluorescence microscopy on cytospin slides. Columns 1–3 represent transmission light images, Hoechst fluores- cence, and PI fluorescence, respectively. Triangles indicate typical early-apoptosic cells and arrows identify characteristic late-apoptotic cells. Data are representative of two independent experiments showing the mean sd from quadruplicate cultures of one experiment.
Gel Electrophoresis Systems, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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model hep 1 panthertm semidry electroblotter  (Thermo Fisher)
98
Thermo Fisher model hep 1 panthertm semidry electroblotter
Figure 3. Oxidized RBC and DSRBC induce apoptosis in J774A.1 MP cells. J774A.1 MP remained untreated (control) or were incubated with oxi- dized RBC or DSRBC at the MP:RBC ratio 1:100. Camptothecin was used as a positive control for apoptosis. After 48 h, cell viability was assayed by TUNEL staining (A and B), <t>DNA</t> migration on agarose gel (C and D), or Hoechst/PI staining (E). (A) Characteristic density plots obtained for control (left panel) and oxidized RBC (MP:RBC ratio 1:100, right panel)-treated J774A.1 MP. R1 gate represents the percentage of TUNEL cells (i.e., apoptotic cells). SSC, Side-scatter. (B) Impact of camptothecin, oxidized RBC, and DSRBC on the percentages of TUNEL J774A.1 MP. (C) DNA profiles of control (Lane 2), oxidized RBC (Lane 3), or camptothecin (Lane 4)-treated J774A.1 MP; Lanes 1 and 5, DNA ladder. (D) DNA profiles of control (Lane 2), DSRBC (Lane 3), or camptothecin (Lane 4)-treated J774A.1 MP. Lanes 1 and 5: DNA ladder. (E) Hoechst/ PI-stained J774A.1 MP analyzed by fluorescence microscopy on cytospin slides. Columns 1–3 represent transmission light images, Hoechst fluores- cence, and PI fluorescence, respectively. Triangles indicate typical early-apoptosic cells and arrows identify characteristic late-apoptotic cells. Data are representative of two independent experiments showing the mean sd from quadruplicate cultures of one experiment.
Model Hep 1 Panthertm Semidry Electroblotter, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ingaas camera model owl 640 swir  (Raptor Photonics)
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Raptor Photonics ingaas camera model owl 640 swir
Figure 3. Oxidized RBC and DSRBC induce apoptosis in J774A.1 MP cells. J774A.1 MP remained untreated (control) or were incubated with oxi- dized RBC or DSRBC at the MP:RBC ratio 1:100. Camptothecin was used as a positive control for apoptosis. After 48 h, cell viability was assayed by TUNEL staining (A and B), <t>DNA</t> migration on agarose gel (C and D), or Hoechst/PI staining (E). (A) Characteristic density plots obtained for control (left panel) and oxidized RBC (MP:RBC ratio 1:100, right panel)-treated J774A.1 MP. R1 gate represents the percentage of TUNEL cells (i.e., apoptotic cells). SSC, Side-scatter. (B) Impact of camptothecin, oxidized RBC, and DSRBC on the percentages of TUNEL J774A.1 MP. (C) DNA profiles of control (Lane 2), oxidized RBC (Lane 3), or camptothecin (Lane 4)-treated J774A.1 MP; Lanes 1 and 5, DNA ladder. (D) DNA profiles of control (Lane 2), DSRBC (Lane 3), or camptothecin (Lane 4)-treated J774A.1 MP. Lanes 1 and 5: DNA ladder. (E) Hoechst/ PI-stained J774A.1 MP analyzed by fluorescence microscopy on cytospin slides. Columns 1–3 represent transmission light images, Hoechst fluores- cence, and PI fluorescence, respectively. Triangles indicate typical early-apoptosic cells and arrows identify characteristic late-apoptotic cells. Data are representative of two independent experiments showing the mean sd from quadruplicate cultures of one experiment.
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Image Search Results


Figure 3. Oxidized RBC and DSRBC induce apoptosis in J774A.1 MP cells. J774A.1 MP remained untreated (control) or were incubated with oxi- dized RBC or DSRBC at the MP:RBC ratio 1:100. Camptothecin was used as a positive control for apoptosis. After 48 h, cell viability was assayed by TUNEL staining (A and B), DNA migration on agarose gel (C and D), or Hoechst/PI staining (E). (A) Characteristic density plots obtained for control (left panel) and oxidized RBC (MP:RBC ratio 1:100, right panel)-treated J774A.1 MP. R1 gate represents the percentage of TUNEL cells (i.e., apoptotic cells). SSC, Side-scatter. (B) Impact of camptothecin, oxidized RBC, and DSRBC on the percentages of TUNEL J774A.1 MP. (C) DNA profiles of control (Lane 2), oxidized RBC (Lane 3), or camptothecin (Lane 4)-treated J774A.1 MP; Lanes 1 and 5, DNA ladder. (D) DNA profiles of control (Lane 2), DSRBC (Lane 3), or camptothecin (Lane 4)-treated J774A.1 MP. Lanes 1 and 5: DNA ladder. (E) Hoechst/ PI-stained J774A.1 MP analyzed by fluorescence microscopy on cytospin slides. Columns 1–3 represent transmission light images, Hoechst fluores- cence, and PI fluorescence, respectively. Triangles indicate typical early-apoptosic cells and arrows identify characteristic late-apoptotic cells. Data are representative of two independent experiments showing the mean sd from quadruplicate cultures of one experiment.

Journal: Journal of leukocyte biology

Article Title: Robust erythrophagocytosis leads to macrophage apoptosis via a hemin-mediated redox imbalance: role in hemolytic disorders.

doi: 10.1189/jlb.0510249

Figure Lengend Snippet: Figure 3. Oxidized RBC and DSRBC induce apoptosis in J774A.1 MP cells. J774A.1 MP remained untreated (control) or were incubated with oxi- dized RBC or DSRBC at the MP:RBC ratio 1:100. Camptothecin was used as a positive control for apoptosis. After 48 h, cell viability was assayed by TUNEL staining (A and B), DNA migration on agarose gel (C and D), or Hoechst/PI staining (E). (A) Characteristic density plots obtained for control (left panel) and oxidized RBC (MP:RBC ratio 1:100, right panel)-treated J774A.1 MP. R1 gate represents the percentage of TUNEL cells (i.e., apoptotic cells). SSC, Side-scatter. (B) Impact of camptothecin, oxidized RBC, and DSRBC on the percentages of TUNEL J774A.1 MP. (C) DNA profiles of control (Lane 2), oxidized RBC (Lane 3), or camptothecin (Lane 4)-treated J774A.1 MP; Lanes 1 and 5, DNA ladder. (D) DNA profiles of control (Lane 2), DSRBC (Lane 3), or camptothecin (Lane 4)-treated J774A.1 MP. Lanes 1 and 5: DNA ladder. (E) Hoechst/ PI-stained J774A.1 MP analyzed by fluorescence microscopy on cytospin slides. Columns 1–3 represent transmission light images, Hoechst fluores- cence, and PI fluorescence, respectively. Triangles indicate typical early-apoptosic cells and arrows identify characteristic late-apoptotic cells. Data are representative of two independent experiments showing the mean sd from quadruplicate cultures of one experiment.

Article Snippet: The DNA pellet was then dissolved in 10 mM Tris-HCl, 10 mM EDTA (pH 8.0), and incubated for 15 min at 45°C, after which, samples were mixed with loading buffer (Invitrogen) and resolved in a 2% agarose gel along with a DNA ladder (FastRuler low-range DNA ladder, Fermentas; Owl separation system, Model B1A, Thermo Scientific Owl, Portsmouth, NH, USA).

Techniques: Control, Incubation, Positive Control, TUNEL Assay, Staining, Migration, Agarose Gel Electrophoresis, Microscopy, Transmission Assay